| Verbruggen, N; Hanikenne, M; Clemens, S: A more complete picture of metal hyperaccumulation through next-generation sequencing technologies., Frontiers Plant Science (2013), doi:10.3389/fpls.2013.00388 [Link] | |
| Abstract: ThemechanisticunderstandingofmetalhyperaccumulationhasbenefittedimmenselyfromtheuseofmoleculargeneticstoolsdevelopedforArabidopsisthaliana.TherevolutioninDNAsequencingwillenableevengreaterstridesinthenearfuture,thistimenotrestrictedtothefamilyBrassicaceae.Referencegenomesarewithinreachformanyecologicallyinterestingspeciesincludingheterozygousoutbreeders.TheywillallowdeepRNA-seqtranscriptomestudiesandthere-sequencingofcontrastingindividualstounravelthegeneticbasisofphenotypicvariation.Cell-typespecifictranscriptomeanalyses,whichwillbeessentialforthedissectionofmetaltranslocationpathwaysinhyperaccumulators,canbeachievedthroughthecombinationofRNA-seqandtranslatomeapproaches.Affordablehigh-resolutiongenotypingofmanyindividualsenablestheelucidationofquantitativetraitlociinintra-andinterspecificcrossesaswellasthroughgenome-wideassociationmappingacrosslargepanelsofaccessions.Furthermore,genome-widescanshavethepowertodetectlociunderrecentselection.Togethertheseapproacheswillleadtoadetailedunderstandingoftheevolutionarypathtowardstheemergenceofhyperaccumulationtraits. |
