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Verbruggen, N; Hanikenne, M; Clemens, S: A more complete picture of metal hyperaccumulation through next-generation sequencing technologies., Frontiers Plant Science (2013) [Link]
Abstract:
ThemechanisticunderstandingofmetalhyperaccumulationhasbenefittedimmenselyfromtheuseofmoleculargeneticstoolsdevelopedforArabidopsisthaliana.TherevolutioninDNAsequencingwillenableevengreaterstridesinthenearfuture,thistimenotrestrictedtothefamilyBrassicaceae.Referencegenomesarewithinreachformanyecologicallyinterestingspeciesincludingheterozygousoutbreeders.TheywillallowdeepRNA-seqtranscriptomestudiesandthere-sequencingofcontrastingindividualstounravelthegeneticbasisofphenotypicvariation.Cell-typespecifictranscriptomeanalyses,whichwillbeessentialforthedissectionofmetaltranslocationpathwaysinhyperaccumulators,canbeachievedthroughthecombinationofRNA-seqandtranslatomeapproaches.Affordablehigh-resolutiongenotypingofmanyindividualsenablestheelucidationofquantitativetraitlociinintra-andinterspecificcrossesaswellasthroughgenome-wideassociationmappingacrosslargepanelsofaccessions.Furthermore,genome-widescanshavethepowertodetectlociunderrecentselection.Togethertheseapproacheswillleadtoadetailedunderstandingoftheevolutionarypathtowardstheemergenceofhyperaccumulationtraits.

last modified 2013-10-01